COBAS HPV TEST


Test information:

The cobas HPV test yields the following three results; one pooled result (types 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, and 66) and 2 individual results (type 16 and 18).


Test Code: HPVDT / CPT Code: 87624 / LOINC Code: 71432-9


Notes:

  1. This test is FDA approved.

Supplies:

  1. ThinPrep Collection Vial
  2. Collection device(s)
    • Endocervical Brush & Plastic Spatula (preferred method)
    • Broom (alternate method)

Collection:
Brush/Spatula Method (preferred)

  1. Obtain an adequate sampling from the ectocervix using a plastic spatula.
  2. Rinse the spatula as quickly as possible into the preservative filled ThinPrep vial.
    • Swirl the spatula vigorously in the vial 10 times.
    • Discard the spatula.
  3. Obtain an adequate sampling from the endocervix using an endocervical brush.
    • Insert the brush into the cervix until only the bottom-most fibers are exposed.
    • Slowly rotate ¼ or ½ turn in one direction – do not over-rotate.
  4. Rinse the brush as quickly as possible in the preservative filled ThinPrep vial.
    • Rotate the device 10 times while pushing against the vial wall.
    • Swirl the brush vigorously to further release material.
    • Discard the brush.
  5. Tighten the cap so that the black line on the cap passes the black line on the vial.

Broom Method (alternate)

  1. Obtain and adequate sampling from the cervix using a broom-like device.
    • Insert the central bristles of the broom into the endocervical canal deep enough to allow the shorter bristles to fully contact the ectocervix.
    • Push gently, and rotate the broom in a clockwise direction 5 times.
  2. Rinse the broom as quickly as possible into the preservative filled ThinPrep vial.
    • Push the broom into the bottom of the vial 10 times, forcing the bristles apart.
    • Swirl the broom vigorously to further release material.
    • Discard the broom.
  3. Tighten the cap so that the black line on the cap passes the black line on the vial.

Check Appropriate Box on Requisition

  1. Some clients may have their own requisition form or an alternate method for ordering HPV tests.
  2. Please call the laboratory if you have any questions on how to order HPV tests at 608-255-5135.

Record Patient Information

  1. Record patient's identification on the ThinPrep vial and on the requisition.
    • A minimum of two identifiers are needed - pre-printed patient labels are preferred.
  2. Place ThinPrep vial and requisition into a transport bag for delivery to the laboratory.

Prepare Specimen for Transport to Laboratory

  1. Place the ThinPrep vial and the requisition into a transport bag for delivery to the laboratory.
  2. Make sure the vial cap is tight and does not leak.

Reason for Rejection:

  1. Unlabeled or mislabeled vial.
  2. Discrepancies between vial and requisition.
  3. Specimen sent by unauthorized person.
  4. Empty vial (e.g. leaky vial).
  5. Contaminated requisition (e.g. from leaky vial).